Transgenic technologies

The goals of transgenic technologies are defined as follows:

• Production of genetically modified mouse and rat models by directed and undirected mutagenesis, using different tools such as CrispR Cas9 or TALEN in zygotes or embryonic stem cells.
• Cryopreservation and the rehabilitation of existing lines
• Import of existing lines via import of live animals or cryopreserved material (spermatozoa, embryos)

An essential strategy in determining the function of genes in medical/biological research is the use of transgenic animal models. The need for new transgenic animal models with defined genetic alterations has increased steadily in recent years. For this reason, the availability of a steadily growing number of transgenic lines makes the establishment of new and efficient transport and import methods indispensable.

The goals of transgenic technologies are defined as follows:

• The goals of transgenic technologies are defined as follows: the production of genetically modified mouse and rat models by non-directed mutagenesis (pronuclear injection, lentiviral mutagenesis) and directed mutagenesis by so-called genome editing (CrispR Cas9, TALEN) in zygotes or embryonic stem cells.
• the cryopreservation and the rehabilitation of existing lines
• the fast and resource-saving import of existing lines available within the scientific community via the import of live animals or cryopreserved material (sperm, embryos)

By combining these techniques, new lines can be produced or imported and bred and maintained under SPF conditions. In addition, a genetic status quo can also be maintained in the long term.