Cryopreservation in the laboratory for transgenic technologies
At the research facilities for experimental medicine (FEM), for research purposes, mouse lines may be
- embryos and also
frozen and thawed again. For cryopreservation, the Transgenic Technologies Laboratory provides quality control of the frozen material to ensure that the lines can be thawed again.
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Archiving and rederivation via cryopreserved embryos and spermatozoa
Both embryos and sperm can be frozen and thawed from mouse lines. Which starting material offers the best chances of success depends, among other things
- from genotype
- the genetic background and
- possible impairments of the animals
and must be clarified in each individual case. If cryopreservation via embryos is necessary, these can be created in vivo or in vitro via IVF.
Cryopreservation includes quality control of the frozen material to ensure that the lines can be thawed again
Since the donors of the embryos are bred in the FEM, we need at least 4 males and 6 to 8 females. For the cryopreservation of the sperm, 2 to 4 males of breeding age (4 to 12 months) are used.
The costs for this service can be found in the current fee schedule of the FEM (please ask at the FEM secretary's office).